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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't nonetheless offered.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.

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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...

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This guide outlines additional feasible optimization of cellular and immunoassay parameters to be certain the best possible outcomes are...

Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.

Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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